Figure 1
From: IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface
Schematic representation of the construction of pMLRM1 and pMLRM2. icsA was amplified from pKMRM1 and was cloned into pBAD33, resulting in pMLRM1. icsA β was amplified from pMLRM1 and was cloned into pBAD33, resulting in pMLRM2. The Not I site (TGCGGCCGC: amino acid 88-91, nucleotide 262-270) of icsA and icsA β serves as the insertion site for bla (TEM-1 beta lactamase) and malE (E. coli maltose binding protein). pBAD is the arabinose inducible promoter, ss represents the IcsA signal peptide, α represents the IcsA passenger domain and β represents the IcsA translocator domain. Drawing is not to scale.