Table 1 Primer sequences for amplification of genes related to β-carotene synthesis, retinal synthesis, and proteorhodopsin
Primer name | Sequence (5'→3') (___: ribosome binding site,:___restriction site) |
|---|---|
crtE forward | GCCCATGG ATGACGGTCTGCGCAAAAAAACACG |
crtE reverse | GCGAATTC TTAACTGACGGCAGCGAGTTTTTTG |
crtB forward | CCGAATTCAAGGAGATATACCA ATGAATAATCCGTCGTTACT CAATCATGC |
crtB reverse | CGGTCGAC CTAGAGCGGGCGCTGCCAGAG |
crtI forward | CCGTCGACAAGGAGATATACAA ATGAAACCAACTACGGTAAT TG |
crtI reverse | CGAAGCTT TCATATCAGATCCTCCAGCATC |
crtY forward | CCAAGCTTGAAGGAGATATACCA ATGCAACCGCACTATGATC TGATTCTC |
crtY reverse | GCCTTAAG TTAGCGATGAGTCGTCATAATGGC |
β-diox forward | GGAGATCTAAGGAGATATACAT ATGGAGATAATATTTGGCCA GAATAAG |
β-diox reverse | CCGGTACC TTAAAGACTTGAGCCACCATGACCC |
pR forward | CCGGTACCAAGGAGATATACAA ATGGGTAAATTATTACTGAT ATTAGGTAG CCGCGGCCGCAAGGAGATATACAA ATGGGTAAATTATTACTGAT ATTAGGTAG |
pR reverse | GCCTCGAG TTAAGCATTAGAAGATTCTTTAACAGCAAC |