Table 3 Oligonucleotides used in this study
From: A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli
oligonucleotides | Sequence |
|---|---|
gfp-F | 5'-GCCATGCATAGTAAAGGAGAAGAACTT-3' |
gfp-R | 5'-GCCCTCGAGCTATTTGTATAGTTCATC-3' |
P5tac-F | 5'-CCCGTCTTACTGTCGGGAATTCGTA-3' |
P5tac-R | 5'-TGCATCTAGTATTTCTCCTCTTTAA-3' |
phaCAB-F | 5'-TTAATGCAT GCGACCGGCAAAGGCGCGGCAGCTTCCAC-3' |
phaCAB-R | 5'-ATTCTCGAG TCAGCCCATATGCAGGCCGCCGTTG-3' |
poxB-F | 5'-GCAGGGGGATTTGGTTCTCGCATAATCGCCTTATGCCCGA TGATATTCCTTTCATCGGGCTACGGCCCCAAGGTCCAAAC-3' |
poxB-R | 5'-GCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCG ATCACTTCATCACCGCGTCTTGGCTTCAGGGATGAGGCG-3' |
RT-PCR | Â |
gapA-F | 5'-AACTGAATGGCAAACTGACTGGTA-3' |
gapA-R | 5'-TTTCATTTCGCCTTCAGCAGC-3' |
phbA-F | 5'-CAAGACCTGGACCTGATGGAG-3' |
phbA-R | 5'-GCCGTTCACATTGACCTTGG-3' |
phbB-F | 5'-GTGGTGTTCCGCAAGATGAC-3' |
phbB-R | 5'-CGACGAGATGTTGACGATGC-3' |
phbC-F | 5'-CTGGACTTTGCCGACAC-3' |
phbC-R | 5'-CGTAGTTCCACACCAGG-3' |
The 5CPtac s promoter cluster's sequence | TTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTG TTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGGAGCTCTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTG |