Efficient one-step production of (S)-1-phenyl-1,2-ethanediol from (R)-enantiomer plus NAD+–NADPH in-situ regeneration using engineered Escherichia coli

Table 1 Enzyme activities and stereoconversions of ( R )-PED ^a to ( S )-enantiomer by recombinant E. coli strains

The stereoconversion of (R)-PED to (S)-PED was performed with the first addition of 1 mM NAD⁺ and 1 mM NADPH, at pH 6.5 and 35°C.
Notes: ^a PED, 1-phenyl-1,2-ethanediol;
^b RCR, (R)-carbonyl reductase, the enzyme activity was measured using (R)-PED as substrate;
^c SCR, (S)-carbonyl reductase, the enzyme activity was measured using 2-hydroxyacetophenone as substrate;
^d PNT, nicotinamide nucleotide transhydrogenase;
^e Mean ± standard deviation;
NT, no detectable enzyme activity.

ISSN: 1475-2859