Table 1 Strains and plasmids used in this study

L. lactis htrA NZ9000

Mutant MG1363 derivative (nisRK genes on the chromosome) lacking htrA

[68]

E. coli TG1

supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK– mK–) [F′ traD36 proAB lacI qZ ΔM15]

ATCC

E. coli BL21 (DE3)

F ^– ompT gal dcm lon hsdS _B (r _B ^- m _B ^- ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Novagen

Plasmid

pET28(b)

Km^r

Novagen

pAW528

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45- CBD-coh1C3-cwa_M6-tlt2

[42]

pAW531

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-cwa_M6-tlt2

[42]

pAW549

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh2O2-cwa_M6-tlt2

This Work

pAW564

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-Lk-coh2O2-cwa_M6-tlt2

This Work

pAW596

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh2O2-Lk-cwa_M6-tlt2

This Work

pAW594

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-Lk-coh2O2-Lk-cwa_M6-tlt2

This Work

pAW546

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh1C3-coh2O2-cwa_M6-tlt2

This Work

pAW561

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh1C3-Lk-coh2O2-cwa_M6-tlt2

This Work

pAW595

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh1C3-coh2O2-Lk-cwa_M6-tlt2

This Work

pAW592

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh1C3-Lk-coh2O2-Lk-cwa_M6-tlt2

This Work

pAW579

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh2S1-cwa_M6-tlt2

This Work

pAW576

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-CBD-coh1C3-coh2S1-cwa_M6-tlt2

This Work

pETdock1

Kn^r; pET28(b)::with cloned dock1 from celS

[42]

pETdock2

Kn^r; pET28(b)::with cloned dock2 from cipA

This Work

pETUdock1

Kn^r; pET28(b)::PT7::6xHis-uidA-dock1

[42]

pETUdock2

Kn^r; pET28(b)::PT7::6xHis-uidA-dock2

This Work

pETU

Kn^r; pET28(b)::PT7::6xHis-uidA

[42]

pETLdock1

Kn^r; pET28(b)::PT7::6xHis-lacZ-dock1

This Work

pETLdock2

Kn^r; pET28(b)::PT7::6xHis-lacZ-dock2

This Work

pETL

Kn^r; pET28(b)::PT7::6xHis-lacZ

This Work