Figure 5

β-glucuronidase and β-galactosidase activities of L. lactis cells with sequential targeting of UidA and LacZ to the scaffold proteins. (A and C) β-glucuronidase and (B and D) β-galactosidase activities resulting from the sequential binding of reporter enzymes onto the chimeric scaffold CBD-coh1C3-coh2S1. (A and B) Sequential targeting of UidA-dock1 and LacZ-dock2 and (C and D) sequential targeting of UidA-dock2 and LacZ-dock1. Enzyme activities are reported for a single enzyme bound to the scaffolds (white bars) and when both enzymes are bound (black bars).