Table 2 Strains and plasmids used in this study

CEN.PK2-1c

MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2

[69]

CEN.PK717.5A

MATa aro3 Δ ARO4^G226S

This study

IMU011

MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2, pUDE172, PYK2::pUDI065

This study

IMK328

MATalpha ura3 his3 leu2 trp1 MAL2-8cSUC2 Δaro3(46,1065)::loxP ARO4^G226S

This study

IMK389

IMK328 pdc6Δ(-6,-2)::loxP pdc5Δ(-6,-2)::loxP

This study

IMK393

IMK389 aro10Δ(-6,-2)::loxP

This study

IMX183

IMK328 pUDE172, PYK2::pUDI065

This study

IMX185

IMK389 pUDE172, PYK2::pUDI065

This study

IMX106

IMK393 pUDE172, PYK2::pUDI065

This study

IMX193

IMK393 pUDE172, PYK2::pUDI065, TRP1::pUDI069

This study

IMX197

IMK393 pUDE172, PYK2::pUDI065, pUDE188

This study

IMX198

IMK393 pUDE172, PYK2::pUDI065, TRP1::pUDI069, pUDE188

This study

Plasmids

pUG6

PCR template for loxp-KanMX4-loxp cassette

[70]

pSH47

Centromeric plasmid, URA3, P_GAL1-Cre- T_CYC1

[70]

pAG416GPD-ccdB

Centromeric plasmid, URA3, P_TDH3-ccdb-T_CYC1, Addgene plasmid 14148

[71]

pAG306GPD-ccdB

Integration plasmid, URA3, P_TDH3-ccdb-T_CYC1, Addgene plasmid 14140

[71]

pAG305GPD-ccdB

Integration plasmid, LEU2, P_TDH3-ccdb-T_CYC1, Addgene plasmid 14138

[71]

pAG304GPD-ccdB

Integration plasmid, TRP1, P_TDH3-ccdb-T_CYC1, Addgene plasmid 14136

[71]

pAG423GPD-ccdB

2 μm ori, HIS3, P_TDH3-ccdb-T_CYC1, Addgene plasmid 14150

[71]

p426TEF

2 μm ori, URA3, P_TEF –T_TEF

[72]

p426TPI

2 μm ori, URA3 P_TPI –T_ADH

[73]

pUNI.U21762

Cloning vector, Ka, atCHS3 cDNA^#

[74]

pUNI.U11924

Cloning vector, Ka, atCHI1 cDNA

[74]

pDZ.4CL3

Cloning vector, Bla, at4CL3 cDNA

[75]

pUD168

pMA cloning vector, Bla, A-P_TDH3-atPAL1-T_CYC1-B^*

This study

pUD169

pMA cloning vector, Bla, B-P_PGI-coCPR1-T_PGI-C

This study

pUD170

pMA cloning vector, Bla, C-P_TPI-coC4H-T_ADH-D

This study

pUD175

pMA cloning vector, Bla, E-P_PGK-coCHS3-T_PGK-F

This study

pUDE185

2 μm ori, HIS3, P_TDH3-coCHS3-T_CYC1

This study

pUDE186

2 μm ori, URA3, P_TEF-coCHS3-T_TEF

This study

pUDE188

2 μm ori, HIS3, P_TDH3-coCHS3-T_CYC1, P_TEF-coCHS3-T_TEF

This study

pUDE103

2 μm ori, URA3, P_TEF-at4CL3-T_TEF

This study

pUDE172

Centromeric plasmid, URA3, P_TDH3-atPAL1-T_CYC1, P_TPI-coC4H-T_ADH, P_PGI-coCPR1-T_PGI

This study

pUDI053

Integration plasmid, LEU2, P_TDH3-atCHI1-T_CYC1

This study

pUDI060

Integration plasmid, LEU2, P_TDH3-atCHI1-T_CYC1, P_TEF-at4CL3-T_TEF

This study

pUDI061

Integration plasmid, LEU2, P_TDH3-atCHI1-T_CYC1, P_TPI-atCHS3-T_ADH, P_TEF-at4CL3-T_TEF

This study

pUDI065

Integration plasmid, LEU2, P_TDH3-atCHI1-T_CYC1, P_TPI-atCHS3-T_ADH, P_TEF-at4CL3-T_TEF, PYK2(1-710)

This study

pUDI069

Integration plasmid, TRP1, P_TDH3-coTAL1-T_CYC1

This study