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Figure 1 | Microbial Cell Factories

Figure 1

From: Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

Figure 1

R-cell construction and temperature-regulated expression of Tel and TelN. A. R-cell construction and temperature-regulated expression of recombinases, Tel and TelN. The cI857-R cassette was inserted into the lacZ gene of E. coli by homologous recombination. Under repressed (25-30°C) conditions, the λ temperature-sensitive CI857 repressor binds to λ operators to inhibit transcription of the recombinase gene, but upon shifting to 40-42°C, the repressor is denatured, falling off the operators and induces expression of the recombinase from the strong λ pL/pR promoter. B. Controllable Tel and TelN expression from R-cells. Expression of the recombinase proteins Tel and TelN under repressed (30°C) and induced (42°C) conditions from total R-cell extract. Lane 1: Weight marker; Lane 2: Recombinase.

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Microbial Cell Factories

ISSN: 1475-2859

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