Table 1 Functional classification and relative fold change of K. oxytoca BAS-10 proteins differentially expressed during anaerobic growth in FEC

1

NADP-specific glutamate dehydrogenase

GLDH

376386651

6.73/49

6.26/45

Amino Acid Metabolism

1

2.6

5^*

Nitrogen regulatory protein P-II 2

GlnK

376394829

5.84/12

5.62/13

Amino Acid Metabolism

−3.2

−8.7

6

Nitrogen regulatory protein P-II 2

GlnK

376394829

5.84/12

5.62/13

Amino Acid Metabolism

1

−2.7

7

Nitrogen regulatory protein P-II 2

GlnK

376394829

5.84/12

5.20/13

Amino Acid Metabolism

1

−2.2

3^*

Ketol-acid reductoisomerase

IlvC

376396684

5.25/54

5.04/54

Amino Acid Metabolism

2.3

2.2

4

D-3-phosphoglycerate dehydrogenase

PHGDH

365906793

6.06/44

5.92/46

Amino Acid Metabolism

1

−2.7

8

Major outer membrane lipoprotein 1

Lpp

376401994

9.36/8

4.16/12

Cell wall

1

3.4

9

Major outer membrane lipoprotein 1

Lpp

376401995

9.36/9

4.04/12

Cell wall

1

3.4

10

Major outer membrane lipoprotein 1

Lpp

376401994

9.36/8

4.44/12

Cell wall

1

1.7

37

Aerobic respiration control protein ArcA

ARCA

376394332

5.30/27

5.04/29

Central carbon metabolism

1.9

1

19^*

Glucose-specific phosphotransferase enzyme IIA component

EIIA^Glc

376399349

4.73/18

4.6/22

Central carbon metabolism

−1.9

−1.7

20^*

Glucose-specific phosphotransferase enzyme IIA component

EIIA^Glc

376399349

4.73/18

4.62/20

Central carbon metabolism

−1.5

−1.4

17^*

Hypothetical protein HMPREF9694_04828 (dihydrolipoyllysine-residue succinyltransferase, E2 component)

SucB

376395176

5.74/44

5.39/54

Central carbon metabolism

−2.8

−3.6

41

Succinyl-CoA synthetase subunit alpha

A-SCS

365909192

5.89/30

5.65/30

Central carbon metabolism

−3.9

1

33

Succinyl-CoA synthetase subunit beta

B-SCS

365909191

5.35/42

5.05/43

Central carbon metabolism

−1.8

1

42

Fumarate hydratase class II

FH

376401593

6.02/50

5.92/46

Central carbon metabolism

1

−2.7

2^*

Fumarate reductase flavoprotein subunit

FrdA

376396036

5.60/66

5.44/77

Central carbon metabolism

2.1

2.6

22^*

Malate dehydrogenase

MDH

376398117

5.57/33

5.34/34

Central carbon metabolism

−3.1

−2.7

23^*

Malate dehydrogenase

MDH

376398117

5.57/33

5.08/29

Central carbon metabolism

−1.9

−2.7

14

Citrate lyase beta subunit

CitE

376376734

5.33/31

4.87/29

Central carbon metabolism

1.9

1

26

Citrate lyase beta subunit

CitE

376376734

5.33/31

5.07/31

Central carbon metabolism

4.9

1

36^*

Citrate lyase beta subunit

CitE

376376734

5.33/31

5.19/29

Central carbon metabolism

3.0

2.4

15^*

Citrate lyase, alpha subunit

CitF

365908401

5.94/55

5.98/55

Central carbon metabolism

8.7

9.0

16^*

Citrate lyase, alpha subunit

CitF

365908401

5.94/55

5.89/55

Central carbon metabolism

14.1

11

24^*

Oxaloacetate decarboxylase alpha chain

OadA

376376739

5.44/63

5.04/64

Central carbon metabolism

10.4

8.7

25^*

Oxaloacetate decarboxylase alpha chain

OadA

376376739

5.44/63

4.7/43

Central carbon metabolism

3.7

2.8

81^*

Oxaloacetate decarboxylase alpha chain

OadA

376376739

5.44/63

4.74/43

Central carbon metabolism

4.0

2.4

29^*

Pyruvate formate lyase

PFL

376393708

5.63/85

5.09/80

Central carbon metabolism

2.7

12.4

30^*

Pyruvate formate lyase

PFL

376393708

5.63/85

5.3/80

Central carbon metabolism

2.1

15.4

31^*

Pyruvate formate lyase

PFL

376393708

5.63/85

5.37/80

Central carbon metabolism

2.9

12.1

32^*

Pyruvate formate lyase

PFL

376393708

5.63/85

5.44/77

Central carbon metabolism

2.7

12.4

35^*

Pyruvate formate lyase

PFL

376393708

5.63/85

5.16/43

Central carbon metabolism

2.3

3.4

27^*

Phosphotrans-acetylase

PTA

365911544

5.26/77

5.27/65

Central carbon metabolism

3.6

2.7

12^*

Acetate kinase A/propionate kinase 2

ACK

365911543

5.84/44

5.39/27

Central carbon metabolism

4.1

1.7

46^*

Triosephosphate isomerase

TIM

376397984

5.82/26

5.5/26

Central carbon metabolism

1.6

1.5

38^*

Pyruvate kinase

PK

365911037

6.00/52

5.78/55

Central carbon metabolism

1.9

1.9

21^*

Glyceraldehyde-3-phosphate dehydrogenase

GAPDH

376401359

6.33/36

6.2/31

Central carbon metabolism

2.2

1.5

28

Pyruvate dehydrogenase subunit E1

PDH

365908482

5.47/100

5.52/67

Central carbon metabolism

2.4

1

40

3-Methyl-2-oxobutanoate hydroxymethyltransferase

PanB

365908516

5.64/28

5.34/29

Cofactor biosynthesis

1

−2.5

49

6,7-Dimethyl-8-ribityllumazine synthase

RibH

365908789

5.12/16

4.87/14

Cofactor biosynthesis

1

−2

51

DNA protection during starvation protein

DPS

376393609

5.72/19

5.61/16

DNA ricombination, replication and repair

−1.5

1

50

DNA-binding protein HU-alpha

HU-2

376396790

9.40/9

9.07/11

DNA ricombination, replication and repair

1

−2.3

52^*

ATP synthase subunit beta

ATPβ

376397178

4.93/50

4.81/51

Energy metabolism

2.5

2.2

53^*

ABC transporter arginine-binding protein 1

ArtJ

376393664

6.90/27

5.98/25

Membrane Transport

4.9

7.4

57^*

Glutamate and aspartate transporter subunit

DEBP

365909131

8.61/33

7.85/30

Membrane Transport

−7.7

−6.7

58^*

Glutamine-binding periplasmic protein

GlnH

376388520

8.74/27

5.81/26

Membrane Transport

−1.9

−2.4

59^*

Glutamine-binding periplasmic protein

GlnH

376388520

8.74/27

7.8/25

Membrane Transport

−3.1

−6.2

60^*

Glutamine-binding periplasmic protein

GlnH

376388520

8.74/27

6.87/25

Membrane Transport

−2.3

−3.8

34^*

Leucine ABC transporter subunit substrate-binding protein LivK

LivK

365907255

5.71/40

5.03/42

Membrane Transport

3.0

3.5

61^*

Maltose ABC transporter periplasmic protein

MBP

365907919

6.88/43

5.96/40

Membrane Transport

−7

−14

56^*

D-galactose-binding periplasmic protein

MGLB

376381367

6.14/36

5.42/29

Membrane Transport

−2.6

−2.5

13

Outer membrane protein A

OmpA

376393752

5.98/38

4.98/31

Membrane Transport

2.7

1

39

Outer membrane protein A

OmpA

376393752

5.98/38

5.5/28

Membrane Transport

1

−1.6

43

Outer membrane protein A

OmpA

376393752

5.98/38

4.78/32

Membrane Transport

1

−1.5

44

Outer membrane protein A

OmpA

376393752

5.98/38

4.86/32

Membrane Transport

1

−1.6

62

Outer membrane protein A

OmpA

376393752

5.98/38

5.17/32

Membrane Transport

1

−2.3

63

Outer membrane protein A

OmpA

376393752

5.98/38

5.03/32

Membrane Transport

1

−3.1

64

Outer membrane protein A

OmpA

376393752

5.98/38

5.39/22

Membrane Transport

1

−5

88

Outer membrane protein A

OmpA

376393752

5.98/38

5.46/27

Membrane Transport

1

−3.7

45

Outer membrane protein W

OmpW

365909926

6.17/23

5.2/22

Membrane Transport

−4.0

1

18^*

Hypothetical protein HMPREF9694_01670 (tricarboxylic transport)

TctC

376400424

8.61/35

6.7/26

Membrane Transport

−6.5

−5.0

48^*

Hypothetical protein HMPREF9694_01670 (tricarboxylic transport)

TctC

376400424

8.61/35

7.8/29

Membrane Transport

−4.1

−8.0

71^*

Hypothetical protein HMPREF9694_01670 (tricarboxylic transport)

TctC

376400424

8.61/35

8.37/29

Membrane Transport

−10

−6.2

65^*

Carbamoyl phosphate synthase small subunit

CPSase

365908404

5.79/42

5.4/42

Nucleotide metabolism

−2

−3.3

66^*

Multifunctional nucleoside diphosphate kinase

NdK

365911711

5.55/15

5.38/14

Nucleotide Metabolism

−2.6

−4.2

67^*

Nucleoside diphosphate kinase

NdKs

376399440

5.55/15

5.42/14

Nucleotide Metabolism

−1.8

−4.1

89

Isochorismatase hydrolase

365909686

5.61/24

5.36/26

Other

−1.6

1

68

Adenosine-3'(2'),5'-bisphosphate nucleotidase

CysQ

365908080

5.67/28

5.31/29

Other

1

−2.1

72

Alkyl hydroperoxide reductase subunit C

AHPC

376395087

5.03/21

4.95/23

Oxido reduction

−2.3

1

76

Superoxide dismutase [Fe]

FeSOD

376402095

5.75/21

5.49/22

Oxido reduction

1

3.1

73

Superoxide dismutase [Mn]

MnSOD

376396893

6.23/23

5.84/25

Oxido reduction

1

−2.6

74^*

Superoxide dismutase [Mn]

MnSOD

376396893

6.23/23

6.01/24

Oxido reduction

−2.8

−6.3

75^*

Superoxide dismutase [Mn]

MnSOD

376396893

6.23/23

5.62/24

Oxido reduction

−2

−4.5

11

Thiol peroxidase

TPX

376401245

4.67/18

4.8/19

Oxido reduction

1

−1.9

79

Chaperonin

CHA10

376396016

5.38/10

4.97/16

Protein metabolism

1

−3.2

82^*

Elongation factor G

EF-G

365907166

5.17/77

5.62/50

Protein metabolism

1.8

1.9

86^*

Ribosomal protein L1

RPL1

376396770

9.56/25

7.77/27

Protein metabolism

−2

−2.2

87^*

Ribosomal protein L1

RPL1

206564980

9.56/25

7.56/28

Protein metabolism

−1.7

−2.1

80

Ribosomal protein L13

RPL13

365907069

9.60/16

8.84/16

Protein metabolism

1

−3.9

85^*

Peptidyl-prolyl cis-trans isomerase SurA

SurA

365908414

6.42/47

5.68/48

Protein metabolism

−2.1

−2.3

78

Autonomous glycyl radical cofactor

GrcA

365911773

4.82/14

4.71/12

Stress response

−1.9

1

69^*

Osmotically-inducible protein Y

OSMY

376376350

8.67/21

5.84/22

Stress response

−3.0

−3.7

47

Universal stress protein F

USF

376401205

5.46/16

5.11/14

Stress response

−1.5

1