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Table 2 Effect of dl -mandelic acid concentration on the biotransformation

From: Rationally re-designed mutation of NAD-independent l-lactate dehydrogenase: high optical resolution of racemic mandelic acid by the engineered Escherichia coli

 

dl-Mandelic acid concentration (g·L-1)

 

10

20

30

40

50

Reaction time (h)

8

18

30

42

54

Benzoylformic acid concentration (g·L-1)

4.8

9.8

14.3

19.1

15.6

Biotransformation efficiency (g·L-1·h-1)

0.62

0.55

0.50

0.47

0.46

Residual l-mandelic acid (g·L-1)

< 0.005

   

9.6

Residual d-mandelic acid (g·L-1)

5.0

10.1

15.1

20.1

25.2

Enantiomeric excess (%)

> 99.5

   

45.0

  1. Values are the mean of 3 separate determinations.