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Table 1 Influence of three harvest methods on the lipolytic activity (EA spec ) fused with three different signal peptides (bpr, amyE, ywmC)

From: An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

 

A: EAspec [kU.g-1] “Over night cultivation”

B: EAspec [kU.g-1] Triggered harvest

C: EAspec [kU.g-1] Triggered induction + harvest

bpr

10.11 ± 2.98

11.84 ± 0.96

13.34 ± 1.25

ywm

3.61 ± 1.50

5.31 ± 0.60

4.40 ± 0.54

amyE

1.22 ± 0.64

1.62 ± 0.33

1.49 ± 0.24

Mean relative error

41%

13%

13%

Duration of stationary phase

6.0 h ± 1.7 h

0.9 h ± 0.4 h

1.6 h ± 0.3 h

  1. Error indicate standard deviation of 9 to 12 fold biological replicates (independent clones). Depending on the sampling method, the mean duration of stationary phase is different. Backscatter data of all processes are provided as supplemental data.
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Microbial Cell Factories

ISSN: 1475-2859

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