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Table 1 Influence of three harvest methods on the lipolytic activity (EA spec ) fused with three different signal peptides (bpr, amyE, ywmC)

From: An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

  A: EAspec [kU.g-1] “Over night cultivation” B: EAspec [kU.g-1] Triggered harvest C: EAspec [kU.g-1] Triggered induction + harvest
bpr 10.11 ± 2.98 11.84 ± 0.96 13.34 ± 1.25
ywm 3.61 ± 1.50 5.31 ± 0.60 4.40 ± 0.54
amyE 1.22 ± 0.64 1.62 ± 0.33 1.49 ± 0.24
Mean relative error 41% 13% 13%
Duration of stationary phase 6.0 h ± 1.7 h 0.9 h ± 0.4 h 1.6 h ± 0.3 h
  1. Error indicate standard deviation of 9 to 12 fold biological replicates (independent clones). Depending on the sampling method, the mean duration of stationary phase is different. Backscatter data of all processes are provided as supplemental data.