Figure 6
Scale- up of C. glutamicum batch culture. Left column: Microtiter cultivation (8 replicates, cells harvested for offline analytic). Middle: 1 L lab-scale bioreactor (2 replicates). Right: 20 L technical scale bioreactor (no replica). Secretory NprE- Cutinase is produced with pEKEx2 vector and 0.5 mM IPTG induction at t = 0. Row A: CDW (black), Glucose (white dots) and lipolytic activity (grey dots) over time. B: lipolytic activity of cutinase over CDW. Slope of regression line represents secretory specific activity EAspec during growth coupled protein expression, C: Specific activity EAspec of cutinase expressed with different signal peptides, error bars is the confidence interval of linear regression (see row B).