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Table 3 Bacterial strains used in this study

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

Strains Endogenous methylation Particular phenotypes Source
  dam dcm   
Escherichia coli     
JM109 + +   New England Biolabs GmbH
BL21 (DE3) + -   New England Biolabs GmbH
GM33 - + dam-3 M. G. Marinus
C2925 - - dcm-6/dam 13::Tn9 New England Biolabs GmbH
Lactic acid bacteria     
L. plantarum CD032    wilde type strain stable grass silage accession number BT6146*
L. plantarum CD033    wilde type strain stable grass silage accession number BT6326*
L. buchneri CD034    wilde type strain stable grass silage accession number BT6327*
E. faecium CD036    wilde type strain stable grass silage accession number BT6329*
L. lactis MG1363    Plasmid free derivative of SH4109 [45]
L. plantarum DSM20174    type strain [39]
  1. * deposited in the strain collection of the Institute of Molecular Biotechnology, Graz, University of Technology, A-8010, Graz, Austria.