Substrate cycles in Penicillium chrysogenum quantified by isotopic non-stationary flux analysis

Table 1 Measured metabolite concentrations and the ratio of intra/extracellular concentrations (assuming a cellular volume of 2.5 mL/gDW)

	IC	IC	EC	ratio
	[μmol/g]	[mM]	[mM]	[−]
TRE	20.4	8.158	0.0204	401
GLC	0.643	0.257	0.0017	151
ICITR	0.041	0.016	0.0001	123
MANOL	175	69.9	0.580	121
CITR	1.69	0.676	0.0061	110
PG2	0.076	0.030	0.0004	82
PG3	0.870	0.348	0.0049	71
MAL	2.50	1.00	0.0179	56
RIB5P	0.739	0.296	0.0054	55
TRE6P	0.201	0.080	0.0015	55
FBP	0.358	0.143	0.0026	54
RBTOL	4.01	1.60	0.0305	53
M6P	1.01	0.404	0.0077	52
SED7P	2.20	0.881	0.0182	48
F6P	0.675	0.270	0.0062	44
G6P	3.05	1.22	0.0288	42
ERYTOL	9.18	3.67	0.115	32
SUCC	0.631	0.252	0.0151	17
DHAP	0.126	0.051	0.0032	16
FUM	0.773	0.309	0.0457	7
AKG	0.576	0.230	0.0403	6
PYR	0.513	0.205	0.0402	5
E4P	0.021	0.008	0.0000	-
PEP	0.155	0.062	ND	ND
PG6	0.055	0.022	ND	ND
G3P	0.357	0.143	ND	ND

ISSN: 1475-2859