Table 6 Primers used in this study
From: One-step generation of error-prone PCR libraries using Gateway® technology
Name | Sequence | Purpose |
|---|---|---|
Nco1-MeV.XD | GGGGCCATGGGCGCATCACGCAGTGTAATCCGCTCC | XD PCR amplification |
MeV.XD-AatII | GGGGGACGTCGACTTCATTATTATCTTCACCAGCAT | |
NTAILF | GGGGACAAGTTTGTACAAAAAAGCAGGCTCTACTACTGAGGACAAGATCAGTAGA | NTAIL PCR amplification |
NTAILR | AGCTTTCTTGTACAAAGTGGTGGATCCCCCC | |
StopNtail | GGGGACAAGTTTGTACAAAAAAGCAGGCTCTTAATAAACTACTGAGGACAAGATCAGTAGA | StopNtail PCR amplification |
XhoI-att R1 (primer 1) | GGGGCTCGAGTACAAGTTTGTACAAAAAAGCTGAA | pNGG construction (Gateway® cassette 5' halve) |
BamHI-mut-R (primer 2) | TCTGGCTTTTAGTAAGCCGGAACCTCTAGATTACGCCCCGCCCTG | |
BamHI-mut-F (primer 3) | CAGGGCGGGGCGTAATCTAGAGGTTCCGGCTTACTAAAAGCCAGA | pNGG construction (Gateway® cassette 3' halve) |
BamHI-att R2 (primer 4) | GGGGGGATCCACCACTTTGTACAAGAAAGCT | |
p17O/ISal1F | GGGGGTCGACGCAGGAATCTCGGAAGAACAAGGC | PCR construction of pDEST17O/I-idNTAIL |
p17O/ISal1R | GGGGGTCGACCGTGTAGAAATGATACTTGGGC | |
T7prom | TAATACGACTCACTATAGG | Sub-cloning screening |
att B2 | CCACTTTGTACAAGAAAGCTGGGT | |
att L1 | TCGCGTTAACGCTAGCATGGATCTC | epPCR |
att L2 | GTAACATCAGAGATTTTGAGACAC |