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Table 3 Effect of the molar ratios of wt NTAIL-PCR (donor) and non-linearized pNGG (acceptor construct).

From: One-step generation of error-prone PCR libraries using Gateway® technology

  Donor Acceptor construct Number of clones
Electroporation 100 fmoles 25 fmoles 0.95 × 107
  100 fmoles 100 fmoles 0.4 × 107
Heat shock 100 fmoles 25 fmoles 0.47 × 105
  100 fmoles 100 fmoles 0.16 × 105
  1. The number of E. coli colonies provided by using two molar ratios of donor and acceptor construct in a 5 μl LR reaction is reported. T7pRos E. coli cells were treated as described in Table 1