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Table 3 Effect of the molar ratios of wt NTAIL-PCR (donor) and non-linearized pNGG (acceptor construct).

From: One-step generation of error-prone PCR libraries using Gateway® technology

 

Donor

Acceptor construct

Number of clones

Electroporation

100 fmoles

25 fmoles

0.95 × 107

 

100 fmoles

100 fmoles

0.4 × 107

Heat shock

100 fmoles

25 fmoles

0.47 × 105

 

100 fmoles

100 fmoles

0.16 × 105

  1. The number of E. coli colonies provided by using two molar ratios of donor and acceptor construct in a 5 μl LR reaction is reported. T7pRos E. coli cells were treated as described in Table 1
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Microbial Cell Factories

ISSN: 1475-2859

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