Figure 2
From: One-step generation of error-prone PCR libraries using Gateway® technology
Schematic of the sub-cloning of a mutated sequence from reporter to non-reporter expression plasmid. From top to bottom, the DNA fragments amplified by PCR between the T7prom and att B2 primers are 1,046 bp, 333 bp, and 560 bp in length, respectively. On the right is illustrated how inverse PCR was used to internally delete 227 bp from the wt NTAIL sequence in pDEST17O/I. Plasmids are not at scale.