Table 3 Sequences of the oligonucleotides used for chromosomal insertions comprobation
Gen | Oligonucleotide | 5′-3′sequence |
|---|---|---|
recA | recA 1 | ATGGGAATTAGCCATGGTCC |
recA | recA 2 | TGTAGGCTGGAGCTGCTTCG |
endA | endA 1 | CGTGGCTGACCAGCTCATCT |
endA | endA 2 | TGCAGGTCGCTTCACGACTC |
nupG | nupG1 | CTTCGCGGATTATCTGCTGA |
nupG | nupG2 | GTGGCAGGATTATCCGACAT |
deoR | deoR1 | GTCCGGTAATGACGCCTGTA |
deoR | deoR2 | CAACGACTTGCCTGTATTGG |