Table 2 Oligonucleotides used in this study
name | confirmation of strain | sequence (5'- > 3') |
|---|---|---|
primers for cloning | ||
MTH1BamHI | GCGATCGCGGATCCTTGAGGAGGTAGGGAACATC | |
MTH1HindIII | CTGACGCCAAGCTTAAACGGCGACTGGTGGTAAG | |
URA3fw | GCTGCTACTCATCCTAGTCC | |
URA3rv | CTTTAGCGGCTTAACTGTGC | |
MTH1fw | CACCATGTTTGTTTCACCACCACCAGCAACTTCG | |
MTH1rv | TCAGGATACTGAATCCGGCTGCCAATCCA | |
primers for diagnostic PCR | ||
MTH1fw | IMI073, IMI076 | CACCATGTTTGTTTCACCACCACCAGCAACTTCG |
MTH1rv | IMI073, IMI076 | TCAGGATACTGAATCCGGCTGCCAATCCA |
m13fw | IMI073, IMI076, IMZ103, IMZ104 | GTAAAACGACGGCCAG |
m132rv | IMI073, IMI076, IMZ103, IMZ104 | GGAAACAGCTATGACCATG |
URA3-A | IMI078, IMI082, IMI083 | TGCATGAGTCTCAGCTCTAC |
URA3-B | IMI078, IMI082, IMI083 | CCAAGGGTAGAGATCCTAAG |