Metabolic flux distribution and Pyk activity. Metabolic flux distribution and Pyk activities of strains W3110 (reference), VH33 (PTS- glc+), VH34 (PTS- glc+pykA-) and VH35 (PTS- glc+pykF-) (from dark to light blue respectively). The figure shows the relative flux of each strain. Between PEP and PYR nodes a graph bar of Pyk activity is depicted. In parenthesis, next to the strains names, the specific glucose consumption rates (mmol / g · h) are shown.