Table 1 Plasmids constructed in this study
Plasmid | Plasmid description |
|---|---|
YEplacp40Cap | wt cap sequence including p40promoter was cloned in YEplac181 vector containing 2μm as replication origin and LEU2 as selection marker |
YEplacRepCap | Entire wt AAV ORFs, rep and cap was cloned in YEplac181 vector containing 2 μm as replication origin and LEU2 as selection marker |
pYESintron Cap | Cap sequence including intron sequence (without p40) located upstream the start codon of VP1 was cloned downstream Gal1 promoter of pYES2 vector. The selection marker is URA3 |
pYESCap | Entire Cap sequence from start codon of VP1 to the VP3 stop codon was cloned downstream Gal1 promoter of pYES2 vector. The selection marker is URA3 |
pYESVP1KM | VP1 coding region was cloned downstream Gal1 promoter of pYES2 vector. yeast Kozak-like element right upstream VP1 start site. Two mutations in major splice acceptor and a mutation in an out of frame ATG. The selection marker is URA3 |