Figure 1

Kinetic profile of the reduction of 4′-chloroacetophenone using free cells for biocatalyst re-use. Reaction conditions: 8 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing 130 mmol/L of isopropanol and 5.0 mmol/L 4′-chloroacetophenone, 0.24 g wet cells, 30°C, 160 rpm. Cycle 1 (□), Cycle 2 (○), Cycle 3 (△), Cycle 4 (▽), Cycle 5 (◇), Cycle 6 (◁), Cycle 7(▷).