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Table 2 Summary of the purification of recombinant Arthrobacter sp. 22c L-arabinose isomerase obtained from 1 L of E. coli BL21(DE3)pLysS culture

From: A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

Purification step Protein (mg) Total activity (U) Specific activity (U mg-1) Purification (fold) Recovery (%)
Cell extract 490 12.76 0.026 1.0 100
Fractogel EMD DEAE 105 11.60 0.110 4.2 21
Fractogel EMD TMAE 48 11.14 0.232 8.9 10
  1. The enzyme activity was measured with D-galactose as a substrate.