From: A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c
Purification step
Protein (mg)
Total activity (U)
Specific activity (U mg-1)
Purification (fold)
Recovery (%)
Cell extract
490
12.76
0.026
1.0
100
Fractogel EMD DEAE
105
11.60
0.110
4.2
21
Fractogel EMD TMAE
48
11.14
0.232
8.9
10