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Table 2 Summary of the purification of recombinant Arthrobacter sp. 22c L-arabinose isomerase obtained from 1 L of E. coli BL21(DE3)pLysS culture

From: A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

Purification step

Protein (mg)

Total activity (U)

Specific activity (U mg-1)

Purification (fold)

Recovery (%)

Cell extract

490

12.76

0.026

1.0

100

Fractogel EMD DEAE

105

11.60

0.110

4.2

21

Fractogel EMD TMAE

48

11.14

0.232

8.9

10

  1. The enzyme activity was measured with D-galactose as a substrate.
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Microbial Cell Factories

ISSN: 1475-2859

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