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Figure 8 | Microbial Cell Factories

Figure 8

From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Figure 8

Comparison of biomass accumulation of three E2 protein expressing strains. Batch fermentation in supplemented YP-lactose medium (3% initial concentration) was performed in a DasGip parallel bioreactor system (DasGip AG, Jülich, Germany) under controlled conditions (see Material and Methods). Biomass accumulation is plotted against process time. VAK726 and VAK834 express the E2-1 allele of BVDV strain CP7, VAK906 contains an E2 allele from BVDV strain NADL[22]. VAK834 and VAK906 carry multiple copies of the KlGAL4 transactivator gene.

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