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Figure 5 | Microbial Cell Factories

Figure 5

From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Figure 5

Comparison of regulated vs. constitutive expression of E2-1 mRNA by Northern analysis in relation to LAC4 mRNA. Total RNA was isolated from cultures grown in 0.5% glucose or lactose as indicated, separated by agarose gel electrophoresis and analyzed by hybridization with Digoxigenin (Roche)-labelled LAC4- or E2-specific RNA probes, respectively. Ethidiumbromide staining of rRNA served as a loading control (top panel).

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Microbial Cell Factories

ISSN: 1475-2859

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