Table 3 Primers used for cassettes and vector construction
From: A novel system for stable, high-level expression from the T7 promoter
Primer name | Sequence (5′ → 3′) |
|---|---|
SalINotI 5'Kan | GGGGTCGACGCGGCCGCAAGGGGTGTTATGAGCCA |
HindIII 3'Kan | AAAAGCTTAGAAAAACTCATCGAGCA |
Tryp1 | TCGACCTAAGCGGCCGCTAATCCCACAGCCGCCAGTTC |
Tryp2 | CGCTGGCGGCATTTT |
Tryp3 | GCGGAACTGGCGGCTGTGGGATTAGCGGCCGCTTAGG |
Tryp4 | GGCCAAAATGCCGCCA |
NdeI 5'E2 | GAGGGCATATGGCACGTCTAGCCTGCAAGGAAGAT |
XhoI 3'E2 | AAAAGCTTCTCGAGTTAGTAGTGTGGGAGTCCGTCAG |
NdeI 5'PA4D | GGGGATTCATATGAAACGTTTTCATTATGATCGCAATAAC |
SalI 3'PA4D | AAAAGCTTCTCGAGTTATCCTATCTCATAGCCTTTTTTAG |
HindIII 3'PA4D | AAAAGCTTATCCTATCTCATAGCCTTTTTTAG |