Growth and plasmid yield of DH5α[pDMB02-GFP] as a function of temperature shift timing. ( A) Growth curves after a temperature shift at OD600 = 0.5 and (B) OD600 = 1.0. (C) Specific yield of pDMB02-GFP after a temperature shift at OD600 = 0.5 and (D) OD600 = 1.0. Data are shown for cultures shifted to 42°C (●) and cultures that remained at 30°C (▵). 0 hr on the x-axis is the time of the temperature shift, and error bars represent the standard deviation calculated from triplicate flasks.