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Figure 3 | Microbial Cell Factories

Figure 3

From: Development of new plasmid DNA vaccine vectors with R1-based replicons

Figure 3

Growth and plasmid yield of DH5α[pDMB02-GFP] as a function of temperature shift timing. ( A) Growth curves after a temperature shift at OD600 = 0.5 and (B) OD600 = 1.0. (C) Specific yield of pDMB02-GFP after a temperature shift at OD600 = 0.5 and (D) OD600 = 1.0. Data are shown for cultures shifted to 42°C () and cultures that remained at 30°C (). 0 hr on the x-axis is the time of the temperature shift, and error bars represent the standard deviation calculated from triplicate flasks.

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