Figure 8
Effect of chaperone co-expression on Tth PAC overproduction in E. coli cells. (A) Western blot against α- and β-Tth PAC subunits. Lane 1 (C), Tth PAC matured in Tth; lane 2, Tth PAC overexpression without chaperone co-expression; lane 3 to 6, co-expression of Tth PAC with GroEL/ES-trigger factor (TF), TF alone, GroEL/ES, and DnaK/J-GrpE, respectively. (B) Optical density at 600nm (dots), specific (white bars) and volumetric (grey bars) activity of Tth PAC in the absence (none) or presence of chaperones (GroEL/ES-TF, TF alone, GroEL/ES or DnaK/J-GrpE).