Figure 4From: Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coliTth inner membrane solubilization and immunodetection of Tth PAC. PAC was immunodetected in Tth HB27 isolated membranes after a 30-min treatment with Sarkosyl at 37°C. T, total membranes; P, insoluble membrane fraction after detergent treatment; SB, fraction of soluble proteins after detergent treatment. SlpA, outer membrane protein marker; Nqo6, inner membrane protein marker.Back to article page