Figure 10From: Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coliHIS 6 :: Tth PAC purification. Fractions of total soluble protein (lane 1), protein flow through (lane 2), 5 mM imidazol protein elution (lane 3), and 150 mM imidazole protein elution were subjected to SDS-PAGE and stained with Coomasie Brillant Blue.Back to article page