Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis

Table 2 Comparison of metabolic profiles of different isobutanol-producing B. subtilis under microaerobic conditions

Strain	Isobutanol production	Isobutanol yield	Lactate	Acetate	Ethanol
	(g/L)	(C-mol/C-mol)	(g/L)	(g/L)	(g/L)
BSUL03	1.95 ± 0.18	0.16 ± 0.01	3.67 ± 022	3.73 ± 0.29	1.09 ± 0.13
BSUL04	2.11 ± 0.15	0.18 ± 0.02	ND	10.65 ± 1.04	1.82 ± 0.27
BSUL05	1.17 ± 0.12	0.36 ± 0.02	ND	ND	ND
BSUL05(+)	2.28 ± 0.17	0.29 ± 0.01	ND	4.46 ± 0.85	ND

Strains were cultured in LBGSM-I medium for 40 h under microaerobic conditions. The plus symbol indicates medium for BSUL05 cultivation is supplemented with 3 g/L sodium acetic acid; ND represents the product is not detected.

ISSN: 1475-2859