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Table 2 Effect of wild type and mutant spores on pH

From: Adsorption of β-galactosidase of Alicyclobacillus acidocaldarius on wild type and mutants spores of Bacillus subtilis

 

n° of sporesa

pHb

Water

0

5.98 ± 0.08

Water + WT spores

2.0 × 109

7.23 ± 0.07

 

1.0 × 1010

7.14 ± 0.24

Water + cotE spores

2.0 × 109

6.06 ± 0.07

 

1.0 × 1010

6.18 ± 0.04

Water + cotH spores

2.0 × 109

5.88 ± 0.07

 

1.0 × 1010

5.85 ± 0.07

  1. a The number of purified spores was measured by direct counting with a Burker chamber under an optical microscope (Olympus BH-2 with 40xlenses); b pH was measured using a pH-meter with a combined glass microelectrode (LLBiotrode, Metrohm) probe in a final volume of 200 μL.
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Microbial Cell Factories

ISSN: 1475-2859

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