Skip to main content

Advertisement

Table 2 Effect of wild type and mutant spores on pH

From: Adsorption of β-galactosidase of Alicyclobacillus acidocaldarius on wild type and mutants spores of Bacillus subtilis

  n° of sporesa pHb
Water 0 5.98 ± 0.08
Water + WT spores 2.0 × 109 7.23 ± 0.07
  1.0 × 1010 7.14 ± 0.24
Water + cotE spores 2.0 × 109 6.06 ± 0.07
  1.0 × 1010 6.18 ± 0.04
Water + cotH spores 2.0 × 109 5.88 ± 0.07
  1.0 × 1010 5.85 ± 0.07
  1. a The number of purified spores was measured by direct counting with a Burker chamber under an optical microscope (Olympus BH-2 with 40xlenses); b pH was measured using a pH-meter with a combined glass microelectrode (LLBiotrode, Metrohm) probe in a final volume of 200 μL.