Table 5 Primers used in this study.
From: Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli
Plasmid | Gene | Primer Sequence (5'-3') |
|---|---|---|
pRJ1 | mgsA | F: GGGAAAGGTACC ATGGCACTTATAATGAATAGTAAAAAAAAGATAGC R: GGGAAAGCATGC TTAAAAATTGTCTTTTCTAATTTTTTGGTAATAAT |
pRJ2 | mgsA | F: GGGAAAGGTACC ATGAAAATTGCTTTGATCGCGCATG R: GGGAAAGCATGC TTATACATTCGGCTCTTCTCCCCGA |
pRJ3 | mgsA | F: GGGAAAGGTACC ATGAATATAGCATTAGTAGCACATGACCAAATGAA R: GGGAAAGCATGC TTAAATACGTTGACTTTTGCTTTTTCTAACTTCTC |
pRJ4 | mgsA | F: GGGAAAGGTACC ATGGAACTGACGACTCGCAC R: GGGAAAGCATGC TTACTTCAGACGGTCCGCGA |
pRJ5 | mgsA | F: GGGAAAGGTACC ATGCCCATGAAGGCCCTGGC R: GGGAAAGCATGC CTATTGGGGGGTTCCCTTGC |
pRJ6 | mgsA | F: GGGAAAGGTACC ATGTGGAATGAAAATATGGAACTGACAACACGTAC R: GGGAAAGCATGC TTATTTCAGGCGCTCGGCAA |
pRJ7 | mgsA | F: GGGAAATGTACA ATGATCGGTATCAGTTTCACCC R: GGGAAAGCATGC TTATCCTCGGCCGGCCAGGTA |
pRJ8 | mgsA | F: GGGAAAGGTACC ATGACTCGCCCCCGCATCGCGTTGAT R: GGGAAATCTAGA TCAGCTGGCCGCCGCTTCGT |
pRJ9 | gldA | F: GGGAAAGCATGC AGGAGATATACC ATGGACCGCATTATTCAATCACCGG R: GGGAAATCTAGA TTATTCCCACTCTTGCAGGAAACGC |
pRJ10 | ydjG | F: GGGAAAGGTACC ATGAAAAAGATACCTTTAGGCACAACGG R: GGGAAATCTAGA TTAACGCTCCAGGGCCTCTGCCATTTCC |
pRJ11 | ydjG budC mgsA | F: GGGAAAGGTACC ATGAAAAAGATACCTTTAGGCACAACGG R: GGGAAAGTCGAC TTAACGCTCCAGGGCCTCTGCCATT F: GGGAAAGTCGAC AGGAGATATACC ATGAAAAAAGTCGCACTTGTTACCGG R: GGGAAACTGCAG TTAGTTAAACACCATCCCGCCGTCG F: GGGAAACTGCAG AGGAGATATACC ATGAAAATTGCTTTGATCGCGCATGAC R: GGGAAATCTAGA TTATACATTCGGCTCTTCTCCCCGA |
pYY93 | ppdABC | F: GGGAAACGTACG ATGAGATCGAAAAGATTTGAAGCACTGGCGAAACG R: GGGAAAAAGCTT TTAATCGTCGCCTTTGAGTTTTTTACGCTCGACG |
pYY109 | budC | F: GGGAAAGGATCC GAAAAAAGTCGCACTTGTTACCGGCG R: GGGAAAGTCGAC TTAGTTAAACACCATCCCGCCGTCG |
pYY134 | gldABC | F: GGGCCCGGTACC ATGAAAAGATCAAAACGATTTGCAGTACTGGCCCA R: GGGCCCAAGCTT TTAGCTTCCTTTACGCAGCTTATGCCGCTGCTGAT |
pYY167 | dhaB12 | F: GGGAAAGGTACC ATGATCAGCAAAGGGTTCAGCACCCAG R: GGGAAAAAGCTT TTATTCCGCGCCTATAGTACACGGAATGCCCATAA |