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Figure 2 | Microbial Cell Factories

Figure 2

From: The acid-tolerant L-arabinose isomerase from the mesophilic Shewanella sp. ANA-3 is highly active at low temperatures

Figure 2

Electrophoretic and size exclusion FPLC analysis of the purified Shewanella enzyme. (A) SDS-PAGE showing the various steps in the purification procedure under reducing conditions. Lane M, protein markers (molecular masses in kDa); lane 1, total cell extract; lane 2, sample from lane 1 after ammonium sulphate precipitation; lane 3, sample from lane 2 after affinity chromatography; lane 4, purified recombinant L-AI after size exclusion chromatography. (B) Size exclusion HPLC analysis of the purified recombinant Shewanella sp. ANA-3 L-AI shows a single peak of 230 kDa (RT = 14.1 min), using protein markers of 669 kDa (RT = 8.755 min), 440 (RT = 13.165 min), 232 (RT = 14.02 min; RT, retention time), 140 (RT = 17.163 min) and 66 (RT = 21.438 min).

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Microbial Cell Factories

ISSN: 1475-2859

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