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Table 4 Activity of P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA, and CCR mutants

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

 

B. subtilis

Treatment (0.2%)

3NA pKAM12

TQ303 pKAM12

MW373 pKAM12

TQ432 pKAM12

3NA pKAM18

TQ303 pKAM18

MW373 pKAM18

Mannitol

13178 ± 1563

6120 ± 1701

11301 ± 628

61 ± 7

83 ± 3

93 ± 4

77 ± 17

Mannitol + Glucose

5920 ± 894

1636 ± 437

11788 ± 550

56 ± 7

19 ± 1

38 ± 3

73 ± 17

Glucose

482 ± 86

287 ± 158

398 ± 137

58 ± 8

12 ± 2

29 ± 1

25 ± 5

Mannitol + Xylose

13693 ± 1343

4982 ± 628

10533 ± 712

51 ± 7

84 ± 8

86 ± 2

74 ± 16

Xylose

555 ± 120

387 ± 32

417 ± 54

44 ± 9

25 ± 3

33 ± 3

25 ± 5

Glucitol

8009 ± 947

3489 ± 854

5211 ± 720

43 ± 5

40 ± 11

68 ± 4

41 ± 9

Glucitol + Glucose

4559 ± 555

774 ± 255

5324 ± 432

37 ± 3

17 ± 8

31 ± 2

40 ± 10

Uninduced

594 ± 83

501 ± 61

433 ± 115

59 ± 7

23 ± 4

33 ± 1

25 ± 6

  1. Expression of lacZ by P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA as well as in CCR affected mutants, such as TQ303 (ΔccpA), TQ432 (ptsH-H15A), and MW373 (ΔptsG) were measured as described in the legend of Table 3.
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Microbial Cell Factories

ISSN: 1475-2859

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