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Table 4 Activity of P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA, and CCR mutants

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

  B. subtilis
Treatment (0.2%) 3NA pKAM12 TQ303 pKAM12 MW373 pKAM12 TQ432 pKAM12 3NA pKAM18 TQ303 pKAM18 MW373 pKAM18
Mannitol 13178 ± 1563 6120 ± 1701 11301 ± 628 61 ± 7 83 ± 3 93 ± 4 77 ± 17
Mannitol + Glucose 5920 ± 894 1636 ± 437 11788 ± 550 56 ± 7 19 ± 1 38 ± 3 73 ± 17
Glucose 482 ± 86 287 ± 158 398 ± 137 58 ± 8 12 ± 2 29 ± 1 25 ± 5
Mannitol + Xylose 13693 ± 1343 4982 ± 628 10533 ± 712 51 ± 7 84 ± 8 86 ± 2 74 ± 16
Xylose 555 ± 120 387 ± 32 417 ± 54 44 ± 9 25 ± 3 33 ± 3 25 ± 5
Glucitol 8009 ± 947 3489 ± 854 5211 ± 720 43 ± 5 40 ± 11 68 ± 4 41 ± 9
Glucitol + Glucose 4559 ± 555 774 ± 255 5324 ± 432 37 ± 3 17 ± 8 31 ± 2 40 ± 10
Uninduced 594 ± 83 501 ± 61 433 ± 115 59 ± 7 23 ± 4 33 ± 1 25 ± 6
  1. Expression of lacZ by P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA as well as in CCR affected mutants, such as TQ303 (ΔccpA), TQ432 (ptsH-H15A), and MW373 (ΔptsG) were measured as described in the legend of Table 3.