Fusion of cre sites to P
(A) and repression of the constructs by glucose (B). (A) Insertion of the cre sites of P
(pKAM89), and of the internal cre site of mtlA (pKAM91), as well as the cre site of acsA, between P
untranslated region. As negative control, P
was directly fused to P
untranslated region (pKAM101). (B) Catabolite repression of B. subtilis 3NA pKAM88, pKAM89, pKAM90, pKAM91, and pKAM101 in the presence and absence of glucose (PTS) and xylose (non-PTS).