Figure 9

Kinetics of rhamnolipid-production in P. putida in a stirred 300 mL reactor. (a) Development of biomass (■) and glucose (▲) concentrations. The experimental data is depicted by symbols, while the lines present the fits using Equations (1) to (3). CDW, cell dry weight. (b) Specific rates characterizing rhamnolipid production in P. putida. The solid line presents the measured ¹³CO₂ production rate originating from ¹³C-labeled glucose. The dashed line represents the glucose uptake rate and was calculated from the fitted glucose concentration curve. The dotted line shows the rate of CO₂ evolution, produced in the rhamnolipid synthesis pathway. The curve was calculated from the experimentally determined yield of rhamnolipid on glucose and the estimated rate of glucose consumption.