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Figure 5 | Microbial Cell Factories

Figure 5

From: Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440

Figure 5

Thin layer chromatography of rhamnolipids. The sample of P. aeruginosa PAO1 (lane 2), grown in PPGAS-medium at 37°C, contains mono- and di-rhamnolipid as does the commercial rhamnolipid (JBR425, Jeneil Biosurfactant Co.) (lane 1). P. putida, expressing the rhlAB operon of P. aeruginosa from the plasmid pVLT33_rhlAB, cultivated in glucose containing LB-medium produced mono-rhamnolipid (lane 4). Empty vector control pVLT33 (lane 3). The band located above the di-rhamnolipids is IPTG (i.e., lanes 3 and 4).

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Microbial Cell Factories

ISSN: 1475-2859

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