Figure 2
From: Optimisation of surface expression using the AIDA autotransporter
Strain dependent expression of protein Z. Comparison of surface expression of protein Z in the wild type and in the OmpT-negative E. coli strain 0:17. (A): Western blot of the outer membrane protein fraction, developed using antiserum against AIDAc. Lane 1: Marker, Lane 2: 0:17, Lane 3: 0:17ΔOmpT, Lane 4: 0:17 with empty vector, Lane 5: 0:17ΔOmpT with empty vector, Lane 6: 0:17 with vector containing protein Z, Lane 7: 0:17ΔOmpT with vector containing protein Z. (B): Cells analysed by flow cytometry using human IgG linked to AlexaFluor488. Red: 0:17 with empty expression vector, Blue: 0:17 with vector containing protein Z, Green: 0:17ΔOmpT with vector containing protein Z.