Table 1 Strains and plasmids used in this study
Strains | Genotype | Reference |
|---|---|---|
A. orientalis HCCB10007 | Vancomycin-producing bacteria | [37] |
S. coelicolor A3(2) M145 | Wild type SCP1- SCP2- | [38] |
R. graminis ATCC20804 | Rhodotorula graminis di Menna, anamorph, mutant derived from KGX39 | ATCC |
DH5α | lacZ ΔM15 endA1 recA1 relA1 gyrA96 deoR nupG λ- | TaKaRa |
JM105 | endA1 glnV44 sbcB15 rpsL thi-1 Δ(lac-proAB) [F' traD36 proAB+ lacIq lacZ ΔM15] hsdR 4 (rK-mK+) | Pharmacia |
BL21(DE3) | F- omp gal dcm lon hsdS B (rB-mB-) λ(DE3) | Novagen |
JW1256-1 | F- λ- ΔlacZ4787 (::rrnB-3), ΔtrpE:: FRT-kan- FRT, rph-1 hsdR514 | CGSC |
N3087 | tyrA16::Tn10 rpsL31(strR) | CGSC |
WT | Wild type E. coli W3110 F- λ- rph-1 INV (rrnD rrnE) | CGSC |
B | W3110 ΔtyrB::FRT | This study |
C | W3110 ΔaspC::FRT | This study |
BC | W3110 ΔtyrB::FRT, ΔaspC::FRT | This study |
BCA | W3110 ΔtyrB::FRT, ΔaspC::FRT, tyrA16::Tn10 | This study |
BCE | W3110 ΔtyrB::FRT, ΔaspC::FRT, ΔtrpE::FRT | This study |
BCAE | W3110 ΔtyrB::FRT, ΔaspC::FRT, tyrA16::Tn10, ΔtrpE::FRT | This study |
Plasmids | Genotype | Reference |
pET24a | kan PT7 expression vector | Novagen |
pEThmaSAo | pET24a derivative, hmaS Ao from A. orientalis | This study |
pET24admd | pET24a derivative, dmd from R. graminis | This study |
pTrc99a | bla P trc lacIq pBR322 ori expression vector | Pharmacia |
pTrc99ahmo | pTrc99a derivative, hmo | This study |
pTrc99aSD | pTrc99a derivative, hmo, dmd | This study |
pKK223-3 | bla P tac pBR322 ori expression vector | Pharmacia |
pKKpheAfbr | pKK223-3 derivative, pheAfbr from E. coli | This study |
pSU2718 | Hae II fragment of pUC18 containing lacZα and MCS, p15A replicon with chloramphenicol acetyl transferase gene (cat) | [39] |
pSUaroFfbr | pSU2718 derivative, aroFfbr from E. coli | This study |
pSUFA | pSU2718 derivative, aroFfbr, pheAfbr | This study |
pSUFAQ | pSU2718 derivative, aroFfbr, pheAfbr, lacIq | This study |
pSUFAAQ | pSU2718 derivative, aroFfbr, pheAfbr, lacIq, hmaS Ao | This study |
pSUFAAQSD | pSU2718 derivative, aroFfbr, pheAfbr, lacIq, hmaS Ao , hmo, dmd | This study |
pIJ790 | araC ParaBAD gam bet exo repA 101ts oriR 101 cat | [33] |
pIJ773 | bla FRT-aac(3)IV-oriT-FRT | [33] |
pIJ778 | bla FRT-aadA-oriT-FRT | [33] |
BT340 | t s bla cat | [33] |