Figure 2
From: Engineering strategy of yeast metabolism for higher alcohol production
Metabolic simulations of multiple deletion mutants generated from the backbone models of E. coli (iBKEco52) and S. cerevisiae (iBKSce50, iBKSce50Δmit, and iBKSce50+7). The product yield and cell growth performance of the proper strains are shown for the production of 1-butanol from AcCoA (a and b), isobutanol from PYR (c and d), and 1-propanol from OAA (e and f). Open circles represent the data obtained from E. coli (iBKEco50); closed red, blue, and orange circles represent the data of original (iBKSce50), merged (iBKSce50Δmit), and expanded (iBKSce50+7) models of S. cerevisiae, respectively. iBKSce50Δmit is an hypothetical S. cerevisiae model derived by merging the cytosolic and mitochondrial networks into one compartment. iBKSce50+7 is a model of iBKSce50 expanded by the addition of 7 E. coli reactions. The cell growth rate data were represented by relative values. A cell growth rate level determined from the wild type model was arbitrary set at 1.