Table 2 Oligonucleotide primers used in this study
Primer | Sequence (5'-3') | Comments |
|---|---|---|
pTX48-F | AGCCCATGG GCGATAAAATTATTCACCTGACT | Forward primer of trxA |
pTX48-R | AGCCTGCAGGATCC CTTGTCGTCGTCGTC ACCAGAAGAATGATGATGATGATGGTG CATATGGCCAGAAC | Reverse primer of trxA flanked by an enterokinase cleavage site and a His-tag |
pTX49-R | AGCCTGCAGGATCC CATATGGCCAGAACCAGAAC | Reverse primer of trxA |
UAL-F | AGCAGCCATGG CAGAACATTTTATAAC | Forward primer of bppU for pNZ8048 |
UAL-R | AGCAGCACTAGT TTAGTGATGGTGATGGTGATG ATTCCGATAAAGTTTTACAATC | Reverse primer of bppL for pNZ8048 |
UALX-F | AGCAGCGGATCC ATGACAGAACATTTTATAAC | Forward primer of bppU for pTX8048 |
UALX-R | AGCAGCACTAGT TTAATTCCGATAAAGTTTTACAATC | Reverse primer of bppL for pTX8048 |
orf40-F | AGCAGCCCATGG GGCGGCTACTAAGTCGCCACTTGCATAAAT | Forward primer of orf40 for pNZ8048 |
orf40-R | AGCAGCACTAGT TTAGTGATGGTGATGGTGATG TAAGTGATAGCCATAAGCAA | Reverse primer of orf40 for pNZ8048 |
orf40X-F | AGCAGCGGATCC ATGGGGCGGCTACTAAGTCGCCACTTGCATAAAT | Forward primer of orf40 for pTX8048 |
orf40X-R | AGCAGCACTAGT TTATAAGTGATAGCCATAAGCAA | Reverse primer of orf40 for pTX8048 |
0140N-F | TGCATCCCATGG ATCATCACCATCACCATCACCATCAC CATCACAGCCGGATTCTCAAGGAC | Forward primer of bbr_0140 for pNZ8048 |
0140N-R | TGCGCATCTAGA TTATGCGATGTAGCTTTC | Reverse primer of orf40 for pNZ8048 |
0140C-F | AATTAACCATGG GCCGGATTCTCAAGGACAAGC | Forward primer of bbr_0140 for pNZ8048 |
0140C-R | TGCCGTTCTAGA TTAGTGATGGTGATGGTGATGGTG ATGGTGATGTGCGATGTAGCTTTCGATGTGTAG | Reverse primer of bbr_0140 for pNZ8048 |
0140X- F | GACAAGGGATCC ATGAGCCGGATTCTCAAG | Forward primer of bbr_0140 for pTX8048 |
0140X-R | AGCTCTCTAGA TTATGCGATGTAGCTTTC | Reverse primer of bbr_0140 for pTX8048 |