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Table 1 Bacterial strains and plasmids used in this study

From: Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system

Strains or plasmids Relevant characteristics Reference or source
Strains   
L. lactis   
NZ9000 MG1363 containing nisRK genes, [3]
NZ9700 expression host of the NICE system
Nisin producing L. lactis strain
[3, 7]
Plasmids   
pNZ8048 Standard L. lactis expression vector, Cmr [3]
pTX8048 pNZ8048 derivative harbouring the TrxA system, contains a His-tag cloned in frame This study
pTX8049 pNZ8048 derivative harbouring the TrxA system This study
pNZ8048-UAL pNZ8048 encoding Tuc2009 bppU, bppA, bppL as an operon This study
pTX8048-UAL pTX8048 encoding Tuc2009 bppU, bppA, bppL as an operon This study
pNZ8048-40 pNZ8048 encoding Tuc2009 orf40 This study
pTX8048-40 pTX8048 encoding Tuc2009 orf40 This study
pNZ8048-0140N pNZ8048 encoding N-terminal His-tagged Bbr_0140 This study
pNZ8048-0140C pNZ8048 encoding C-terminal His-tagged Bbr_0140 This study
pTX8048-0140 pTX8048 encoding Bbr_0140 This study
  1. Cm, chloramphenicol.