Table 1 Bacterial strains and plasmids used in this study
Strains or plasmids | Relevant characteristics | Reference or source |
|---|---|---|
Strains | ||
L. lactis | ||
NZ9000 | MG1363 containing nisRK genes, | [3] |
NZ9700 | expression host of the NICE system Nisin producing L. lactis strain | |
Plasmids | ||
pNZ8048 | Standard L. lactis expression vector, Cmr | [3] |
pTX8048 | pNZ8048 derivative harbouring the TrxA system, contains a His-tag cloned in frame | This study |
pTX8049 | pNZ8048 derivative harbouring the TrxA system | This study |
pNZ8048-UAL | pNZ8048 encoding Tuc2009 bppU, bppA, bppL as an operon | This study |
pTX8048-UAL | pTX8048 encoding Tuc2009 bppU, bppA, bppL as an operon | This study |
pNZ8048-40 | pNZ8048 encoding Tuc2009 orf40 | This study |
pTX8048-40 | pTX8048 encoding Tuc2009 orf40 | This study |
pNZ8048-0140N | pNZ8048 encoding N-terminal His-tagged Bbr_0140 | This study |
pNZ8048-0140C | pNZ8048 encoding C-terminal His-tagged Bbr_0140 | This study |
pTX8048-0140 | pTX8048 encoding Bbr_0140 | This study |