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Figure 3 | Microbial Cell Factories

Figure 3

From: Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system

Figure 3

Co-production of the Tuc2009 baseplate components BppU, BppA and BppL. (A) Protein gel analysis of soluble fractions of NZ9000 + pTX8048-UAL without nisin induction (lane1), NZ9000 + pNZ8048-UAL following induction with 0.2% (v/v) nisin (lane 2) and NZ9000 + pTX8048-UAL following induction with 0.2% (v/v) nisin (lane 3). (B) Protein gel of Ni-affinity purified baseplate complex from NZ9000 + pTX8048-UAL soluble fraction. Lanes: L, prestained marker; 1, flow-through; 2-3, elution fractions.

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Microbial Cell Factories

ISSN: 1475-2859

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