Production of a small phage protein encoded by Tuc2009 ORF40. (A) Protein gel analysis of soluble fractions of NZ9000 + pTX8048-40 without nisin induction (lane 1), and after induction with 0.2% (v/v) nisin (lane 2), soluble fraction of NZ9000 + pNZ8048-40 after 0.2% nisin induction (lane 3). (B) Protein gel of purified Tuc2009 ORF40 from NZ9000 + pTX8048-40. Lanes: L, prestained marker; 1-3, elution fractions. (C) Immunoblotting analysis showing Tuc2009 ORF40 using mouse anti-polyhistidine antibody as primary antibody. The Tuc2009 ORF40 protein product is indicated by an arrow.