Figure 3
Comparison of amount of Enfuvirtide peptide (mg/gdw) produced in the different B. halodurans strains containing pSECFliCFFuz. An ELISA plate assay using Anti-FLAG antibodies was used to determine the concentration of fusion protein produced in the culture supernatants of the different strains at selected time points. The amount of Enfuvirtide peptide was calculated and presented as mg Enfuvirtide per grams dry weight. The error bars show the ± SD of three separate experiments.