Figure 5
Serum analysis from E2-T1 inoculated mice. (A) ELISA analysis of mice receiving three injections of E2-T1. Mice (n = 4) were injected with 50 μg E2-T1 and 10 μg QuilA at 2 week intervals. ELISA assays were performed using pre-immune sera and sera obtained two weeks following each injection, termed 1st, 2nd and terminal samples. (B) Western blot analysis of E2-T1 with terminal sera. Mice sera was used at the following dilutions: lane 1, 1:4000; lane 2, 1:8000; lane 3, 1:16000; lane 4, 1:32000; lane 5, 1:64000; lane 6, VMRD monoclonal 348; lane 7, VMRD monoclonal D89; lane 8, VMRD monoclonal 157.