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Figure 3 | Microbial Cell Factories

Figure 3

From: Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates

Figure 3

Protein fractions following endotoxin removal from E2-T1 IB by Triton X-114 extraction were separated by electrophoresis on 10% Bis-Tris gel and stained with Coomassie blue. Lane 1, SeeBlue® Plus2 MW standards; lane 2, IB pellets resuspended in PBS before Triton X-114 treatment; lane 3, aqueous layer post Triton X-114 treatment; lane 4, recovered IB pellet post Triton X-114 treatment; lane 5, Triton TX-114 layer.

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Microbial Cell Factories

ISSN: 1475-2859

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