Figure 9

Antigenicity of the expressed recombinant GST-opt-VP1 protein using CAV-infected chicken serum and Western blot analysis (A). Lane M, pre-stained protein marker; lane 1, 2 and 3, purified GST-opt-VP1 protein was respectively recognized by anti-GST mAb, anti-VP1 mAb E3 and CAV-infected positive chicken serum. Reactivity of the chicken serum with the recombinant GST-opt-VP1 protein was also evaluated by indirect ELISA (B). Five groups of CAV-infected positive chicken sera and one group of negative sera samples were used in a GST-opt-VP1 based ELISA. Five sera samples are involved in each group. These sera had been all identified as negative or positive using a commercial ELISA kit purchased from the IDEXX laboratory.