Table 1 Effect of some additives, substrate chain length and temperature on the activity of LipC12

Cation (1 mM) after EDTA chelation^a

Cation (1 mM) without EDTA chelation

Substrate specificity

   None

   < 0.5

   None^b

   100.0 ± 3.4

   pNP palmitate (C16:0)^b,d

   100.0 ± 2.2

   CaCl₂^b

   100.0 ± 1.2

   RbCl

   128.4 ± 2.3

   pNP myristate (C14:0)

   75.4 ± 1.7

   CuCl₂

   59.3 ± 0.3

   KCl

   121.8 ± 4.6

   pNP dodecanoate (C12:0)

   90.0 ± 4.9

   Al₂(SO₄)₃

   59.2 ± 0.1

   CsCl

   104.1 ± 4.7

   pNP decanoate (C10:0)

   83.2 ± 1.7

   CoCl₂

   54.9 ± 0.6

   NaCl

   94.3 ± 4.4

   pNP caproate (C6:0)

   29.3 ± 0.7

   MnCl₂

   54.9 ± 1.2

   LiCl

   92.7 ± 2.3

   pNP valerate (C5:0)

   34.3 ± 2.1

   NiCl₂

   53.3 ± 0.6

   Ba(Ac)₂

   85.4 ± 5.3

   pNP butyrate (C4:0)

   25.6 ± 0.8

   FeSO₄

   37.4 ± 0.9

   CaCl₂

   73.4 ± 4.2

   pNP acetate (C2:0)

   1.9 ± 0.2

   Pb(Ac)₂

   33.9 ± 0.2

   SnCl₂

   67.6 ± 3.4

Optimal temperature ^e

   CdSO₄

   32.7 ± 0.3

   NiCl₂

   66.0 ± 1.0

   9.9°C

   38.4 ± 6.4

   HgCl₂

   29.5 ± 0.9

   MgCl₂

   64.5 ± 1.9

   20.5°C

   93.8 ± 6.6

   MgCl₂

   4.0 ± 0.1

   HgCl₂

   62.3 ± 1.3

   30.0°C^b

   100.0 ± 5.1

   AgNO₃

   0.9 ± 0.2

   CoCl₂

   53.8 ± 0.7

   40.0°C

   37.7 ± 7.7

   Ba(Ac)₂

   0.8 ± 0.1

   CuCl₂

   52.4 ± 0.9

   50.7°C

   14.8 ± 3.5

   KCl

   < 0.5

   CdSO₄

   49.8 ± 2.3

   60.6°C

   11.1 ± 3.6

   ZnCl₂

   < 0.5

   FeCl₃

   48.8 ± 5.3

Sodium chloride

   SnCl₂

   < 0.5

   MnCl₂

   47.1 ± 1.7

   None^b

   100.0 ± 2.9

   LiCl

   < 0.5

   ZnCl₂

   44.5 ± 2.9

   0.01 M

   109.4 ± 4.5

   RbCl

   < 0.5

   Al₂(SO₄)₃

   44.8 ± 1.3

   0.05 M

   121.8 ± 2.0

   CsCl

   < 0.5

   FeSO₄

   33.4 ± 0.5

   0.1 M

   126.0 ± 3.2

   NaCl

   < 0.5

   Pb(Ac)₂

   26.9 ± 0.4

   0.2 M

   148.8 ± 4.1

   FeCl₃

   < 0.5

   AgNO₃

   8.2 ± 0.4

   0.5 M

   349.4 ± 26.1

Anion^c (1 mM)

Anion^c (10 mM)

   1.0 M

   1346.5 ± 38.2

   None^b

   100.0 ± 4.5

   None^b

   100.0 ± 1.4

   1.5 M

   1501.5 ± 74.6

   CH₃COO-

   108.4 ± 7.8

   SO₄²-

   110.4 ± 0.9

   2.0 M

   1333.5 ± 95.4

   NO₃-

   102.6 ± 2.6

   SO₃²-

   109.1 ± 1.0

   2.5 M

   37.2 ± 7.5

   SO₄²-

   99.3 ± 3.4

   CH₃COO-

   108.4 ± 2.2

   3.0 M

   114.3 ± 1.6

   Cl -

   97.4 ± 5.4

   Cl -

   104.5 ± 0.8

   3.5 M

   164.4 ± 8.1

   SO₃²-

   96.9 ± 3.5

   NO₃-

   102.2 ± 2.7

   4.0 M

   170.9 ± 1.7

   PO₄³-

   93.2 ± 6.2

   PO₄³-

   68.0 ± 1.4

Modifying reagent (1 mM)

Detergent (0.1%)

Detergent (1%)

   None^b

   100.0 ± 0.6

   None^b

   100.0 ± 3.7

   None^b

   100.0 ± 1.2

   PMSF

   13.1 ± 1.2

   CTAB

   175.0 ± 3.7

   Triton X-100

   34.3 ± 0.3

   DEPC

   35.4 ± 0.4

   NLS

   130.5 ± 0.1

   NP40

   29.0 ± 0.5

Chelating agent (10 mM)

   Triton X-100

   114.1 ± 3.6

   NLS

   16.9 ± 0.4

   None^b

   100.0 ± 1.5

   NP40

   102.8 ± 2.6

   Tween 40

   15.4 ± 0.7

   EDTA

   50.0 ± 2.4

   Tween 20

   56.4 ± 1.8

   Tween 20

   15.4 ± 0.2

   EGTA

   22.4 ± 0.6

   SDS

   50.7 ± 2.4

   Tween 80

   12.9 ± 0.3

Emulsifying agent

   Tween 40

   38.6 ± 1.0

   CTAB

   12.0 ± 0.7

   None^b

   100.0 ± 2.2

   Tween 80

   32.6 ± 0.3

   SDS

   8.8 ± 0.2

   Gum arabic (0.5%)

   186.5 ± 1.3